Mitsuko Horie (Japanese: 堀江美都子, Hepburn: Horie Mitsuko, born March 8, 1957) is a Japanese actress, voice actress and singer from Yamato, Kanagawa. She is known as the "Queen of the Anison World" (アニソン界の女王, Anison-kai no Joō).

Horie has voiced several characters throughout her career, such as Sailor Galaxia in Sailor Moon Sailor Stars and Remi in the Nippon Animation World Masterpiece Theater series Remi, Nobody's Girl. She is also part of the judging panel at the Animax Anison Grand Prix, with fellow singers Ichirou Mizuki, and Yumi Matsuzawa. As a voice actress, she is contracted to Aoni Production.