The Harvard University Choir, more commonly referred to as the University Choir or simply UChoir, is Harvard University's oldest choir. It has provided choral music for the Harvard Memorial Church and its predecessor church for over 180 years, and is Harvard's only professional choir. Each year, a select group of choristers also make up the Harvard Choral Fellows, who sing at the church's daily Morning Prayers service in Appleton Chapel.

The University Choir is the only professional choir on campus. Singers are paid a significant stipend each year. The Choir is directed by Edward Elwyn Jones, the Gund University Organist and Choirmaster at Memorial Church, and David von Behren, the Assistant University Organist and Choirmaster. In fall 2009, UChoir performed in the 100th Carols Services, the oldest carols service in the country, and in that spring performed J.S. Bach's St. John's Passion.