Daphne Rubin-Vega (née Vega; born November 18, 1969) is an American actress, dancer, and singer-songwriter. She is best known for originating the roles of Mimi Marquez in the 1996 premiere of the Broadway musical Rent and Lucy in the 2007 premiere of the Off-Broadway play Jack Goes Boating.

Rubin-Vega also appeared as Bombshell publicist Agnes in the second season of the TV series Smash (2012) and as Luisa Lopez in the TV series Katy Keene (2020). In 2021, Rubin-Vega starred as salon owner Daniela in the film adaptation of Lin-Manuel Miranda's In the Heights. In 2024, she voiced Carmilla Carmine in the adult animated musical animated series Hazbin Hotel.